4ZG1

Crystal structure of a nanobody raised against KDM5B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	110
Detector technology	CCD
Collection date	2014-12-12
Detector	MAR CCD 165 mm
Wavelength(s)	1
Spacegroup name	P 43 2 2
Unit cell lengths	91.520, 91.520, 184.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.206 - 1.850
R-factor	0.1884
Rwork	0.186
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3EZJ chain A
RMSD bond length	0.009
RMSD bond angle	1.156
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.210	41.210	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.110	0.044	0.670
Rmeas	0.118
Rpim	0.043	0.020	0.306
Total number of observations	510190	10913	5674
Number of reflections	67728
<I/σ(I)>	12.2	25.5	24.6
Completeness [%]	100.0	98.9	99.8
Redundancy	7.5	6.3	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	279	50mM Hepes, 150mM Nacl, 0,133 M NH4NO3 14% PEG4000