4ZFV
Lipomyces starkeyi levoglucosan kinase bound to ADP and magnesium.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-30 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.399, 114.399, 232.499 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.200 - 1.500 |
R-factor | 0.1584 |
Rwork | 0.158 |
R-free | 0.17420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yh5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.095 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.200 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.099 | 0.787 |
Number of reflections | 244767 | |
<I/σ(I)> | 10.3 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | Equal volumes of reservoir buffer (22% polyethylene glycol (PEG) 4000, 0.1 M sodium acetate, 0.1 M Tris pH 7.5) and LGK (7 mg/ml) in crystallization buffer (50 mM NaCl, 2 mM ADP, 4 mM MgCl2 , 0.5 mM TCEP, 20 mM Tris pH 7.5) were mixed. |