4ZFU
Structural studies on a non-toxic homologue of type II RIPs from Momordica charantia (bitter gourd) in complex with N-acetyl D galactosamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 144.380, 136.330, 44.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.010 - 2.530 |
R-factor | 0.20171 |
Rwork | 0.199 |
R-free | 0.24383 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z8s |
RMSD bond length | 0.013 |
RMSD bond angle | 1.578 |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.124 | 2.670 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.800 | |
Number of reflections | 30178 | |
<I/σ(I)> | 10.1 | 2.2 |
Completeness [%] | 98.8 | 92.9 |
Redundancy | 6.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1 M HEPES, 20% w/v PEG 10000, Glycerol |