4ZFM
Structure of Gan1D-E170Q in complex with cellobiose-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.590, 97.470, 105.270 |
| Unit cell angles | 90.00, 97.66, 90.00 |
Refinement procedure
| Resolution | 22.370 - 1.400 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ze5 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.074 | 0.542 |
| Number of reflections | 398539 | |
| <I/σ(I)> | 10.7 | 3 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16% PEG 8K, 3% MPD, 0.1 M imidazole buffer pH 6.5 |
