4ZEY

Crystal structure of a nuclear receptor binding factor 2 MIT domain (NRBF2) from Homo sapiens at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.95369,0.97968,0.97943
Spacegroup name	C 1 2 1
Unit cell lengths	48.480, 34.050, 52.340
Unit cell angles	90.00, 108.47, 90.00

Refinement procedure

Resolution	27.366 - 1.500
R-factor	0.2107
Rwork	0.209
R-free	0.24820
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XSCALE (November 3, 2014 BUILT=20141118)
Phasing software	SOLVE
Refinement software	BUSTER-TNT (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.366		1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.092	0.057	0.392
Rmeas	0.128	0.080	0.538
Total number of observations	39652
Number of reflections	12873	2341	2234
<I/σ(I)>	7.56	20.4	1.8
Completeness [%]	92.4	91.6	92.1
Redundancy	1.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25.0% Glycerol, 1.50M ammonium sulfate