4ZEY
Crystal structure of a nuclear receptor binding factor 2 MIT domain (NRBF2) from Homo sapiens at 1.50 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.95369,0.97968,0.97943 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.480, 34.050, 52.340 |
| Unit cell angles | 90.00, 108.47, 90.00 |
Refinement procedure
| Resolution | 27.366 - 1.500 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.24820 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (November 3, 2014 BUILT=20141118) |
| Phasing software | SOLVE |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.366 | 1.550 | |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.092 | 0.057 | 0.392 |
| Rmeas | 0.128 | 0.080 | 0.538 |
| Total number of observations | 39652 | ||
| Number of reflections | 12873 | 2341 | 2234 |
| <I/σ(I)> | 7.56 | 20.4 | 1.8 |
| Completeness [%] | 92.4 | 91.6 | 92.1 |
| Redundancy | 1.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25.0% Glycerol, 1.50M ammonium sulfate |
