4ZEM
Crystal structure of eIF2B beta from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918410 |
| Spacegroup name | H 3 |
| Unit cell lengths | 138.000, 138.000, 146.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.458 - 2.550 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The N-terminal domain (res. 34-134) of chain A in PDB entry 2YVK and the C-terminal domain (res. 107-305) of chain B in entry 3ECS were used. |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.154 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 | |
| High resolution limit [Å] | 2.540 | 50.000 | 2.540 |
| Rmerge | 0.050 | 0.584 | |
| Rmeas | 0.058 | 0.677 | |
| Total number of observations | 134799 | ||
| Number of reflections | 34537 | 3801 | |
| <I/σ(I)> | 23.71 | 2.62 | |
| Completeness [%] | 99.9 | 99.9 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 100 mM HEPES, 1.33 M tri-sodium citrate |
