4ZE5
Structure of Gan1D-E170Q, a catalytic mutant of a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.550, 97.440, 105.240 |
| Unit cell angles | 90.00, 97.82, 90.00 |
Refinement procedure
| Resolution | 27.640 - 1.480 |
| R-factor | 0.13346 |
| Rwork | 0.132 |
| R-free | 0.15929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ZEH |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.504 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.067 | 0.375 |
| Number of reflections | 337040 | |
| <I/σ(I)> | 12.6 | 3.9 |
| Completeness [%] | 99.7 | 94.9 |
| Redundancy | 5.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-18% PEG 8K, 3% MPD, 0.1 M imidazole buffer pH 6.5 |
