4ZE4
Structure of Gan1D, a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-12-01 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.010, 68.880, 152.880 |
| Unit cell angles | 90.00, 100.74, 90.00 |
Refinement procedure
| Resolution | 31.900 - 1.920 |
| R-factor | 0.19612 |
| Rwork | 0.194 |
| R-free | 0.24519 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.783 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.030 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.104 | 0.323 |
| Number of reflections | 86922 | |
| <I/σ(I)> | 6 | 2.3 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 17% PEG 8K, 3% MPD, 0.1 M imidazole buffer pH 6.5 |
