4ZC0
Structure of a dodecameric bacterial helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2013-04-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2546 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 283.467, 283.467, 283.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.245 - 6.700 |
| R-factor | 0.2597 |
| Rwork | 0.258 |
| R-free | 0.29880 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.610 | 7.060 |
| High resolution limit [Å] | 6.700 | 6.700 |
| Rmerge | 0.177 | 4.725 |
| Number of reflections | 6960 | |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 44.3 | 45.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | Polyethylene glycol 3350 26%, 100 mM Tris pH 8.6 and 200 mM Lithium Sulphate |
