4ZBV
Structural studies on a non-toxic homologue of type II RIPs from Momordica charantia (bitter gourd) in complex with benzyl T-antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 130.310, 138.400, 44.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.620 - 2.000 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z8s |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.874 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.279 | 1.246 |
| Number of reflections | 56031 | |
| <I/σ(I)> | 6.6 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1 M HEPES, 25% w/v PEG 10000, No-cryo protectant used |
