4ZBM

Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	78
Detector technology	CCD
Collection date	2008-02-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 4
Unit cell lengths	67.907, 67.907, 89.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
Rwork	0.204
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k0d
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	15224
<I/σ(I)>	19	0.97
Completeness [%]	95.0
Redundancy	6.5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	65%MPD, 100mM KCl, 100mM MES, 4mM n-Decyl-beta-D-Maltoside