4ZBL
Phototoxic fluorescent protein mKillerOrange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 64.160, 64.160, 47.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.800 - 1.570 |
| R-factor | 0.1458 |
| Rwork | 0.144 |
| R-free | 0.17520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gb3 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.242 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
| High resolution limit [Å] | 1.570 | 3.380 | 1.570 |
| Rmerge | 0.041 | 0.023 | 0.240 |
| Rmeas | 0.047 | 0.026 | 0.284 |
| Rpim | 0.024 | 0.013 | 0.150 |
| Total number of observations | 115789 | ||
| Number of reflections | 29879 | ||
| <I/σ(I)> | 12.4 | ||
| Completeness [%] | 98.1 | 99.3 | 96 |
| Redundancy | 3.9 | 3.9 | 3.5 |
| CC(1/2) | 0.999 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 0.09M citric acid, pH 3.5, 22.5% PEG 3350 |
