4ZB7
Phanerochaete chrysosporium URE2P6 in apo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.363, 84.888, 92.779 |
| Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.400 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zb8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.246 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.674 |
| Number of reflections | 37186 | |
| <I/σ(I)> | 20.9 | 3.3 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 10.1 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 278 | 25 % PEG4000, 0.1M Sodium Acetate pH4.6, 0.2M Ammonium Sulfate |
