4ZB5
A form of glucose isomerase collected at 100K.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.904, 98.152, 102.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.900 - 2.000 |
| R-factor | 0.11679 |
| Rwork | 0.115 |
| R-free | 0.15408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mnz |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.011 |
| Data reduction software | xia2 (xia2/dials) |
| Data scaling software | xia2 (xia2/dials) |
| Phasing software | MOLREP (11.3.01) |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.900 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.068 |
| Number of reflections | 31979 | |
| <I/σ(I)> | 18.7 | 15.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Hepes, 10% PEG 400, 0.05 M Manganese Chloride, 20% Glucose |
