4ZAD
Structure of C. dubliensis Fdc1 with the prenylated-flavin cofactor in the iminium form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.970, 64.550, 96.050 |
| Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
| Resolution | 67.040 - 2.460 |
| R-factor | 0.20761 |
| Rwork | 0.205 |
| R-free | 0.25569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.716 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.040 | 2.520 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rpim | 0.091 | 0.480 |
| Number of reflections | 39186 | |
| <I/σ(I)> | 6.7 | 1.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium thiocyanate, sodium acetate pH 5.5 and 15% w/v PEG 4000. |
