4ZAC
Structure of S. cerevisiae Fdc1 with the prenylated-flavin cofactor in the iminium form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.410, 96.180, 116.640 |
| Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
| Resolution | 54.190 - 1.650 |
| R-factor | 0.16437 |
| Rwork | 0.163 |
| R-free | 0.19231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4za4 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.467 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.190 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.039 | 0.392 |
| Number of reflections | 282233 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | crystals were obtained in 0.2M calcium acetate, 20% PEG 3000 |
