4Z9J
Apo-Tar from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2014-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.410, 73.510, 79.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.830 - 1.780 |
| R-factor | 0.18455 |
| Rwork | 0.182 |
| R-free | 0.23418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2asr |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.974 |
| Data reduction software | iMOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.832 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.062 | |
| Number of reflections | 34426 | |
| <I/σ(I)> | 19.1 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | NaCl |
