4Z9H
Asp-Tar from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.320, 59.260, 79.230 |
| Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
| Resolution | 24.570 - 1.452 |
| R-factor | 0.17497 |
| Rwork | 0.173 |
| R-free | 0.20497 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2asr |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.350 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.570 | 1.530 |
| High resolution limit [Å] | 1.452 | 1.452 |
| Rmerge | 0.055 | |
| Number of reflections | 65392 | |
| <I/σ(I)> | 11.1 | 3.1 |
| Completeness [%] | 98.8 | 95.9 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | NaCl |
