4Z8I
Crystal structure of Branchiostoma belcheri tsingtauense peptidoglycan recognition protein 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97886 |
| Spacegroup name | P 65 |
| Unit cell lengths | 77.206, 77.206, 82.607 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.603 - 2.701 |
| R-factor | 0.1732 |
| Rwork | 0.172 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oht |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.812 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.101 | |
| Number of reflections | 15022 | |
| <I/σ(I)> | 11 | 2.9 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 1.2 M Lithium sulfate, 0.1 M Tris-HCl |
