4Z89
SH3-II of Drosophila Rim-binding protein bound to a Cacophony derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.300, 122.220, 68.540 |
| Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
| Resolution | 43.868 - 2.640 |
| R-factor | 0.2582 |
| Rwork | 0.255 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z88 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.136 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.166 | 0.710 |
| Number of reflections | 25229 | |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 96.9 | 95 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 100 mM MES pH 6.0, 200 mM Calciumacetate, 20% PEG 8000 |
