4Z7N

Integrin alphaIIbbeta3 in complex with AGDV peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	256.883, 144.373, 104.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.189 - 2.599
R-factor	0.2304
Rwork	0.230
R-free	0.25270
RMSD bond length	0.006
RMSD bond angle	0.493
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2104: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.599
Number of reflections	139660
<I/σ(I)>	6.51
Completeness [%]	97.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	277	11% PEG 8000, 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K