4Z6G

Structure of NT domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-22
Detector	ADSC QUANTUM 1
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	94.088, 94.088, 192.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.175 - 2.654
R-factor	0.2101
Rwork	0.208
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f7p 2odv
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	SHELXL
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.600
Number of reflections	27500
<I/σ(I)>	4.6
Completeness [%]	98.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	MES, (NH4)2SO4, (NH4)2HPO4