4Z64

the plant peptide hormone receptor complex in arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-09
Detector	BRUKER SMART 6500
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	152.539, 220.888, 105.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.913 - 2.659
R-factor	0.2024
Rwork	0.200
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z5w
RMSD bond length	0.008
RMSD bond angle	1.265
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.650
Number of reflections	49253
<I/σ(I)>	15.5
Completeness [%]	96.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium acetate pH4.5, 2.0 M (NH4)2SO4