4Z63

The plant peptide hormone receptor in arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-09
Detector	BRUKER SMART 6500
Wavelength(s)	1
Spacegroup name	P 43 2 2
Unit cell lengths	92.892, 92.892, 242.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.544 - 2.514
R-factor	0.231
Rwork	0.229
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4z5w
RMSD bond length	0.008
RMSD bond angle	1.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.500
Number of reflections	36369
<I/σ(I)>	26
Completeness [%]	98.4
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Bis-Tris 5.5, 2.0M (NH4)2SO4