4Z4R
Crystal structure of GII.10 P domain in complex with 300mM fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.984463 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.104, 78.938, 68.889 |
| Unit cell angles | 90.00, 99.84, 90.00 |
Refinement procedure
| Resolution | 43.435 - 1.801 |
| R-factor | 0.1413 |
| Rwork | 0.140 |
| R-free | 0.17310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3onu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.433 |
| Data reduction software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.440 | 1.866 |
| High resolution limit [Å] | 1.801 | 1.801 |
| Rmerge | 0.040 | 0.171 |
| Number of reflections | 64070 | |
| <I/σ(I)> | 21.9 | 6.59 |
| Completeness [%] | 99.2 | 95.78 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium nitrate, bis-tris propane, PEG3350 |
