4Z3P
MATE transporter ClbM in complex with Rb+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.220, 80.220, 175.251 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.099 - 3.300 |
| R-factor | 0.2069 |
| Rwork | 0.198 |
| R-free | 0.28690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vvn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.133 | |
| Number of reflections | 10052 | |
| <I/σ(I)> | 5.8 | 2.3 |
| Completeness [%] | 97.3 | 97.9 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.8 | 293 | 30% PEG 500 DME, 100 mM lithium sulfate, 400 mM rubidium chloride, 100 mM sodium cacodylate |
