4Z3J
Crystal structure of the lectin domain of PapG from E. coli BI47 in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99986 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.760, 56.560, 70.940 |
| Unit cell angles | 112.76, 102.76, 88.12 |
Refinement procedure
| Resolution | 52.068 - 2.500 |
| R-factor | 0.2207 |
| Rwork | 0.219 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j8s |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.853 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.068 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.310 |
| Number of reflections | 23326 | |
| <I/σ(I)> | 8.5 | 2.5 |
| Completeness [%] | 95.2 | 95.5 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30 % PEG2000 MME, 0.15 M KBr |
