4Z2Z
New crystal structure of yeast Ddi1 aspartyl protease reveals substrate engagement mode
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.515, 50.051, 131.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.18438 |
| Rwork | 0.183 |
| R-free | 0.21305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.478 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.060 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.036 | 0.127 |
| Number of reflections | 25235 | |
| <I/σ(I)> | 25.3 | 7 |
| Completeness [%] | 96.3 | 79.4 |
| Redundancy | 4.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295 | 0.1M phosphate-citrate, pH 4.2, 0.4M NaCl, 20% PEG 8000, 8 mg/ml protein, 1:1 uL drop |
