4Z2V
Crystal structure of short hoefavidin-hoef-peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 60.740, 60.740, 166.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.070 - 1.390 |
R-factor | 0.16161 |
Rwork | 0.160 |
R-free | 0.18659 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z27 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.510 |
Data reduction software | MOSFLM |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.070 | 1.430 |
High resolution limit [Å] | 1.390 | 1.390 |
Number of reflections | 61114 | |
<I/σ(I)> | 19.4 | |
Completeness [%] | 95.0 | |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M NaCl, 0.1 M Bis-Tris pH 6.2-7.0 and 1.1-1.5 M ammonium sulfate |