4Z26
Mimivirus R135 (residues 51-702)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.318, 240.503, 295.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.909 - 2.915 |
| R-factor | 0.1676 |
| Rwork | 0.165 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z24 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.909 | 2.990 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.182 | 0.674 |
| Number of reflections | 158141 | |
| <I/σ(I)> | 8.4 | 2.3 |
| Completeness [%] | 96.2 | 84.3 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 8000, ammonium formate, 1-n-Butyl-3-methylimidazolium n-octylsulfate |
