4Z24
Mimivirus R135 (residues 51-702)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.760, 77.500, 94.340 |
| Unit cell angles | 111.22, 109.64, 94.56 |
Refinement procedure
| Resolution | 47.707 - 2.000 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q9t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.087 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.209 | 0.992 |
| Number of reflections | 105150 | |
| <I/σ(I)> | 6.3 | 1.6 |
| Completeness [%] | 99.2 | 99.3 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | HEPES, PEG 8000, calcium chloride |
