4Z05
C. bescii Family 3 pectate lyase mutant E84A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-06 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 35.270, 145.570, 160.077 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.500 |
R-factor | 0.1533 |
Rwork | 0.152 |
R-free | 0.18641 |
RMSD bond length | 0.027 |
RMSD bond angle | 2.394 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 66566 | |
<I/σ(I)> | 17.29 | 2.71 |
Completeness [%] | 99.7 | 98.4 |
Redundancy | 6.42 | 3.22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8.4 and 66.8% MPD |