4YZL

Crystal structure of the indole prenyltransferase TleC complexed with indolactam V and DMSPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-21
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	42.380, 74.100, 112.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.717 - 2.096
R-factor	0.1912
Rwork	0.189
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yzj
RMSD bond length	0.009
RMSD bond angle	1.182
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.096
Number of reflections	21405
<I/σ(I)>	22.73
Completeness [%]	99.8
Redundancy	7.21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100mM MES-NaOH (pH6.5) 1700mM (NH4)2SO4