4YZJ

Crystal structure of selnomethionin-labeled indole prenyltransferase TleC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-21
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9788
Spacegroup name	P 21 21 21
Unit cell lengths	41.870, 73.920, 110.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.234 - 2.106
R-factor	0.1905
Rwork	0.188
R-free	0.23490
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.211
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((phenix.refine: 1.8.2_1309))
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.106
Number of reflections	38054
<I/σ(I)>	15.37
Completeness [%]	99.4
Redundancy	7.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100mM MES-NaOH (pH6.5), 1785mM (NH4)2SO4