4YYM
Crystal structure of TAF1 BD2 Bromodomain bound to a butyryllysine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.215, 66.048, 81.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.700 - 1.500 |
| R-factor | 0.2203 |
| Rwork | 0.218 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eqf |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.178 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.051 | 0.038 | 0.946 |
| Total number of observations | 302465 | ||
| Number of reflections | 50570 | ||
| <I/σ(I)> | 12.4 | ||
| Completeness [%] | 99.3 | 99.1 | 97.7 |
| Redundancy | 6 | 5.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M calcium chloride, 20 w/v PEG3350 |
