4YY8
Crystal Structure Analysis of Kelch protein from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-29 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.189, 117.020, 74.566 |
| Unit cell angles | 90.00, 113.88, 90.00 |
Refinement procedure
| Resolution | 40.780 - 1.810 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4in4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.966 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.658 |
| Number of reflections | 89721 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.3 | 66.6 |
| Redundancy | 4.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG 3350, Sodium azide |
