4YY6
Crystal structure of BRD9 Bromodomain bound to a butyryllysine peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.799, 35.070, 30.157 |
| Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
| Resolution | 26.830 - 1.450 |
| R-factor | 0.1813 |
| Rwork | 0.180 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hme |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.491 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 3.120 | 1.450 |
| Rmerge | 0.075 | 0.058 | 0.270 |
| Total number of observations | 70650 | ||
| Number of reflections | 22761 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 95.1 | 97.2 | 70.3 |
| Redundancy | 3.1 | 3.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 0.2 M sodium malonate, pH 4.0, 20% w/v PEG3350 |
