4YY4
Crystal structure of BRD9 Bromodomain bound to DMSO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.145, 68.546, 105.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.590 - 1.470 |
| R-factor | 0.2178 |
| Rwork | 0.217 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hme |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.764 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 3.170 | 1.470 |
| Rmerge | 0.053 | 0.039 | 0.452 |
| Total number of observations | 123026 | ||
| Number of reflections | 21295 | ||
| <I/σ(I)> | 10.6 | ||
| Completeness [%] | 96.5 | 99 | 80.8 |
| Redundancy | 5.8 | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES, pH 7.5, 25% w/v PEG3350 |
