4YXJ
Structure of Thermotoga maritima DisA in complex with ApCpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00149 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 108.250, 108.250, 166.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.411 - 2.550 |
| R-factor | 0.1926 |
| Rwork | 0.190 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c1z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.133 | 0.618 |
| Number of reflections | 32830 | |
| <I/σ(I)> | 15.5 | 2.1 |
| Completeness [%] | 99.2 | 93.9 |
| Redundancy | 5.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 30% (v/v) MPD, 200mM Ammonium acetate, 100 mM TRis-HCl |
