4YVI

Crystal Structure of H. influenzae TrmD in complex with sinefungin and tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-29
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	86.770, 86.770, 227.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.529 - 3.010
R-factor	0.2215
Rwork	0.218
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yvh 3akz
RMSD bond length	0.008
RMSD bond angle	1.387
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	17898
<I/σ(I)>	20.7
Completeness [%]	99.6
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium acetate trihydrate, ammonium phosphate monobasic, PEG 20000