4YVF
Structure of S-adenosyl-L-homocysteine hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-03 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.82565 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.528, 134.150, 185.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.810 - 2.700 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23900 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 31605 |
| <I/σ(I)> | 25.4 |
| Completeness [%] | 99.6 |
| Redundancy | 6.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES, PEG 4000, 2-propanol, ethyl acetate |
