4YUD

Crystal structure of a RNA binding motif protein 39 (RBM39) from Homo sapiens at 1.28 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	86.574, 26.711, 35.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.461 - 1.280
R-factor	0.1487
Rwork	0.146
R-free	0.19420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cq4
RMSD bond length	0.015
RMSD bond angle	1.698
Data reduction software	XDS (March 30, 2013)
Data scaling software	XSCALE (March 30, 2013)
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.461	32.461	1.350
High resolution limit [Å]	1.280	4.050	1.280
Rmerge		0.013	0.285
Rmeas	0.027	0.014	0.355
Rpim	0.013	0.007	0.205
Total number of observations	77481	2929	4520
Number of reflections	20012
<I/σ(I)>	27.7	82.2	3
Completeness [%]	92.2	98.2	63
Redundancy	3.9	3.8	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% polyethylene glycol 3350, 0.20M sodium fluoride, 1mM RNA-6b UAAUAA