4YTZ
Rat xanthine oxidoreductase, C-terminal deletion protein variant, crystal grown without dithiothreitol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.172, 139.123, 223.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.300 |
| R-factor | 0.2098 |
| Rwork | 0.207 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3an1 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.889 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.082 | 0.047 | 0.368 |
| Total number of observations | 468751 | ||
| Number of reflections | 135086 | ||
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 97.0 | 94 | 98.5 |
| Redundancy | 3.5 | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEGl 8000, lithium formate, sodium salicylate, EDTA, glycerol, HEPES |
