4YTW
Crystal structure of Ups1-Mdm35 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-06-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.750, 71.746, 87.607 |
| Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
| Resolution | 29.230 - 1.400 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.077 | 0.824 |
| Number of reflections | 103802 | |
| <I/σ(I)> | 41.6 | 2.2 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 7.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M ammonium citrate pH 7.0, 20% PEG 3350 |
