4YSW

Structure of rat xanthine oxidoreductase, C-terminal deletion protein variant, NADH bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	98.460, 137.643, 222.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.410 - 1.990
R-factor	0.18303
Rwork	0.181
R-free	0.21881
RMSD bond length	0.009
RMSD bond angle	1.205
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.410
High resolution limit [Å]	1.990
Number of reflections	195400
<I/σ(I)>	33.6
Completeness [%]	94.7
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	PEG 8000, lithium formate, DTT, sodium salicylate, EDTA, glycerol, HEPES