4YSQ
Structure of copper nitrite reductase from Geobacillus thermodenitrificans - 8.38 MGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.75000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 114.951, 114.951, 84.225 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.350 - 1.500 |
| R-factor | 0.14266 |
| Rwork | 0.140 |
| R-free | 0.18732 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.908 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 64868 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 96.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M sodium acetate, 5.5%(w/v) PEG 4000, 0.075M CuSO4 |
