4YSL

Crystal structure of SdoA from Pseudomonas putida in complex with glutathione

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Collection date	2014-01-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 43
Unit cell lengths	79.625, 79.625, 93.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.459 - 1.462
R-factor	0.177
Rwork	0.176
R-free	0.20370
RMSD bond length	0.014
RMSD bond angle	1.546
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.460
Number of reflections	100527
<I/σ(I)>	30.6
Completeness [%]	98.2
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	200 mM Ammonium acetate, 100 mM HEPES (pH 7.5), and 25% (w/v) PEG 3350