4YSK

Crystal structure of apo-form SdoA from Pseudomonas putida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Collection date	2013-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99988
Spacegroup name	P 43
Unit cell lengths	79.505, 79.505, 94.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.626 - 2.466
R-factor	0.2185
Rwork	0.214
R-free	0.26230
RMSD bond length	0.002
RMSD bond angle	0.641
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	20723
<I/σ(I)>	42.143
Completeness [%]	99.7
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	200 mM Ammonium acetate, 100 mM HEPES (pH 7.5), and 25% (w/v) PEG 3350