4YSE

High resolution synchrotron structure of copper nitrite reductase from Alcaligenes faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-05
Detector	RIGAKU SATURN A200
Wavelength(s)	0.95000
Spacegroup name	P 21 21 21
Unit cell lengths	61.871, 101.149, 146.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.650 - 1.200
R-factor	0.13118
Rwork	0.129
R-free	0.16799
RMSD bond length	0.029
RMSD bond angle	2.506
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.200
Number of reflections	281187
<I/σ(I)>	22.9
Completeness [%]	98.2
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	100mM sodium acetate, pH 4, 8% PEG4000