4YS4
Crystal structure of Pf41 tandem 6-cys domains from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 133.760, 133.760, 133.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.299 - 2.450 |
| R-factor | 0.1844 |
| Rwork | 0.182 |
| R-free | 0.22790 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.746 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.290 | 2.570 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.078 | 0.573 |
| Number of reflections | 14643 | |
| <I/σ(I)> | 18.2 | 3 |
| Completeness [%] | 99.0 | 92.8 |
| Redundancy | 10.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.01 M zinc chloride, 0.1 M MES buffer pH 6.0, 20% PEG6000 |
